Infrastructure
The infrastructure of the research center has been intensively developed in the last years, through the financial support of several national and international research projects of the research group members. Furthermore several instruments were obtained by donations from our long-time collaborators, Terapia Ranbaxy, Farmec, Kynetix.
The infrastructure is organized over several platforms:
- Recombinant protein expression and purification Platform – L.6 and L.36
- Protein engineering Platform - L.32 and L.33
- Enzyme technologies Platform - L.53, L.11, and L.16)
- Bioanalytical Platform - L.35 and L.25
- Protein crystallization Platform - L.9
- Computational facilities
The research center also has access to the strategic research facilities of the Faculty of Chemistry and Chemical Engineering of Babes-Bolyai University (NMR spectrometers. Atomic absorption-emission spectrometer, etc.).
Regulation for the use of biotransformation and biocatalysis research center equipment.
Recombinant protein expression and purification Platform
Includes facilities for:
A. protein expression in various bacterial hosts: deep-freezers, autoclaves, biosafety laminar hoods, differently sized incubators, 4-L bioreactor, differently sized-centrifuges and ultracentrifuge sonicator, French-press, cold-room.
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Deep-freezer Premium U410 Ultra-Low Temperature
Manufacturer: New Brunswick 2014
-81 °C, volume of 80 L.
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Raypa AES-40 autoclave
Manufacturer: Raypa Lab. Equipment 2007
40 L, with various predefined sterilisation programs.
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Presoclav 80 autoclave
Manufacturer: Selecta 2016
40 L, with various predefined sterilisation programs.
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GFL Water Still
Manufacturer: Jena 2015
System for double-distilled water with a production capacity of 2 l/h.
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Incubator Multi Stack Shaking Labtech
Manufacturer: Daihan Labtech India Pvt. Ltd. 2007
100-300 rpm, 25-70°C, 10-2000 mL glassware.
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Incubator Innova 40 R
Manufacturer: New Brunswick 2016
25-500 rpm, temperature range 25-70 °C, 10-2000 mL
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Bioreactor MS-F1
Manufacturer: Bionet Engineering Co 2010
Autoclavable 4L bioreactor, equipped with pums, filters, specific sensors / electrodes and software for control.
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Himac CR30NX High-Speed Refrigerated Centrifuge with P70AT Angle Rotors
Manufacturer: Himac/Eppendorf
Speeds up to 110,000 x g, suitable for ultracentrifugation, with a broad application range: continuous flow to separation steps of small particles such as viruses, organelles, extracellular vesicles or nanoparticles, etc.
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B. for protein purification: peristaltic pumps, Äkta-FPLC protein purification system with diverse sets of columns: affinity, desaltion, ion-exchange, size-exclusion with both sizes of analytical and preparativ applications, gel-electrophoresis and western-blotting systems, imaging system for gel and blott documentation.
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Protein purification system FPLC-Äkta
Manufacturer: GE Health Care 2007
Pump with two channels, detector UV 900, conductometer, fraction collector Frac-950, Unicorn software.
Technical description
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Horizontal and vertical electrophoresis systems with gel-casting
Manufacturer: Biorad 2015, 2016
Preparation and migration of 1-4 gels used for the analysis of proteins and nucleic acids.
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Lyophilizer - ALPHA 1-2 LDPlus
Manufacturer: Martin Christ 2007
Equipped with high-vacuum (< 0.040 atm) pump
Technical description
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Milli-Q EQ 7000 Ultrapure Water Purification System
Manufacturer: Merck
Flow rate up to 2 L/min; <0.001EU/ml endotoxin removal capability.
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