Infrastructure
The infrastructure of the research center has been intensively developed in the last years, through the financial support of several national and international research projects of the research group members. Furthermore several instruments were obtained by donations from our long-time collaborators, Terapia Ranbaxy, Farmec, Kynetix.
The infrastructure is organized over several platforms:
- Recombinant protein expression and purification Platform – L.6 and L.36
- Protein engineering Platform - L.32 and L.33
- Enzyme technologies Platform - L.53, L.11, and L.16)
- Bioanalytical Platform - L.35 and L.25
- Protein crystallization Platform - L.9
- Computational facilities
The research center also has access to the strategic research facilities of the Faculty of Chemistry and Chemical Engineering of Babes-Bolyai University (NMR spectrometers. Atomic absorption-emission spectrometer, etc.).
Regulation for the use of biotransformation and biocatalysis research center equipment.
Bioanalytical Platform
Includes facilities for:
A. monitoring biochemical reaction by LC-ESI-MS techniques, reaction monitoring by liquid (normal-, reverse-phased) and/or gas-chromatography with more > 100 columns, including >30 chiral columns for enantipurity assessments.
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Agilent LC-MS system
Manufacturer: Agilent Technologies Inc. 2006
Analysis and characterization of compounds, inclusive from a complex mixture (biological samples). Equipped with specific software and databases as well as the necessary auxiliar instrumentations.
Technical description
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Gas chromatograph GC Agilent MF 235330
Manufacturer: Agilent Technologies Inc. 2009
FID detector, autosampler, Chemstation software –equipped with diverse standard and chiral columns.
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Chiral chromatographic analysis unit
HPLC Agilent 1150 (2002)
- Quaternary pump, degasor
- DAAD detector
- Chemstation software
- autosampler
HPLC Agilent 1200 (2006)
- Quaternary pump, degasor
- DAAD detector
- Chemstation software
HPLC Agilent 1260 (2012)
- Quaternary pump, degasor,
- DAAD detector
- ELSD detector (with N2 generator)
- Chemstation software
A collection of standard and chiral columns (> 30).
Technical description: 1150, 1200 and 1260 model.
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Titrator 902 Titrando – pH STAT
Manufacturer: Methrom 2012
pH measurements, automated titration, pH monitoring and automated adjustment, 3 point calibration, etc.
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ADP220 Digital polarimeter
Manufacturer: Bellingham Stanley 2005
with cuvettes of 1,2 and 10 ml.
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B. Protein-ligand binding assessments by UV-, fluorescence spectroscopy, fluorescent polarisation and/or isothermal calorimeter.
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Affinity ITC autosampler
Manufacturer: TA Instruments 2020
The Isothermal titration calorimetry (ITC) allows the determination of binding constants, reaction stoichiometry, and total reaction energy of two or more compounds (small or large molecules) in solution, by measuring the heat that is released or absorbed when molecules interact with each other.
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UV-VIS Agilent 8453 spectrophotometer
Manufacturer: Agilent Technologies Inc. 2010
Analysis in UV-VIS domain, equipped with thermostat, multi-channel sample system with 8 positions and specific software including enzyme kinetics and other applications.
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RF-6000 Spectrofluorophotometer
Manufacturer: Shimadzu
Scanning Wavelength range 200 to 900 nm; Resolution 1.0 nm or less.
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