Infrastructure

The infrastructure of the research center has been intensively developed in the last years, through the financial support of several national and international research projects of the research group members. Furthermore several instruments were obtained by donations from our long-time collaborators, Terapia Ranbaxy, Farmec, Kynetix.

The infrastructure is organized over several platforms:

  1. Recombinant protein expression and purification Platform – L.6 and L.36
  2. Protein engineering Platform - L.32 and L.33
  3. Enzyme technologies Platform - L.53, L.11, and L.16)
  4. Bioanalytical Platform - L.35 and L.25
  5. Protein crystallization Platform - L.9
  6. Computational facilities

The research center also has access to the strategic research facilities of the Faculty of Chemistry and Chemical Engineering of Babes-Bolyai University (NMR spectrometers. Atomic absorption-emission spectrometer, etc.).

Regulation for the use of biotransformation and biocatalysis research center equipment.

Enzyme technologies Platform


Includes facilities for:
A. Enzyme immobilisation: development and synthesis of diverse solid supports, support characterization support functionalization, derivatization, enzyme immobilisation via adsorption, encapsulation or covalent binding: rotavapors, chemical hoods, diverse small shaker incubators, electrospinning instruments, zeta-potential analyser, transmission electron microscope TEM.

Electrospinning system FLUIDNATEK

Manufacturer: Bioinicia
A precise electrospinning and electrospraying system to produce nanofibers and particles with tightly controlled diameters and morphologies. It offers fully adjustable parameters including emitter and collector voltages, flow rate, automated needle translation, spinning distance, and more for optimized nanomaterial production.

Technical description

Transmission electron microscope TEM Hitachi H-7650

Manufacturer: Hitachi Group 2010
Registration of high resolution and contrast images, necessary for analytical, biomedical, materials sciences.

Technical description

B. Development of enzyme-based Lab-on-Chip systems, optimized within microfluidic instruments, including in-house chip-development.

Flow chemistry system - SpinSplit

Manufacturer: SpinSplit Technical Research and Development LLC

Technical description

C. Synthesis of diverse small molecules, serving as substrates, inhibitors or diverse probes (fluorescent, linkers) for the investigated enzyme reactions: 2 synthesis labs with 4 chemical hoods, 4 rotavaps, UV-lamps, 1 flash chromatography systems, preparative HPLC.

Chemical hoods

Flash chromatography system

Hei-VAP Core - hand lift model with G3 vertical glassware

Manufacturer: Heidolph

D. Synthesis of diverse small molecules, serving as substrates, inhibitors or diverse probes (fluorescent, linkers) for the investigated enzyme reactions: 2 synthesis labs with 4 chemical hoods, 4 rotavaps, UV-lamps, 1 flash chromatography systems, preparative HPLC.

Vapourtec Flow chemistry – RS-600 Multiple step reactions

Manufacturer: Vapourtec 2013
A multi-pump, multi-reactor system where telescoping reactions and multi-step, multi treatment reactions can be performed with ease in an automated controlled manner. Supplied with two R4 reactors, three R2C+ pump modules, R-Series software and autosampler. The system is used in industry and academia.

Technical description