ABSTRACT:
The main objective
of the present proposal is to achieve new enzymatic DKR procedures for the enantioselective synthesis of both enantiomers of alfa- and beta-aryl-alanines,
aryl-amines and aryl-hydroxy-propanoates
using batch and continuous flow procedures. For the successful execution of the
project the biocatalytic potential of several lipases
will be investigated. Moreover, the selection strains which could produce
hydrolases with opposite selectivity will be taking into consideration. The
production, purification, characterization and immobilization of such kind of (S)-selective enzymes together with other
know (R)-selective lipases will allow
the elaboration of efficient batch DKR procedures. The found optimal conditions
will be the prerequisite for building various types of continuous flow
procedures for a more efficient production of various enantiopure
chiral building blocks.
