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Novel lipases with usual and opposite selectivity (anti-Kauzlauskas’ rule) immobilized in different forms,
encapsulated whole cell ketoreductase systems with
various stereoselectivity as
"off-the-self" tools will be developed and different chiral
building blocks will be prepared. We are planning to test 5-8 different
immobilization techniques that may influence and enhance the productivity,
stability, enantiomer selectivity of the applied biocatalyst.The
application of enzyme immobilization technologies makes the bioconversation economically feasible and comparable to
the synthetic solutions therefore increases the economical
benefits. Finally, we plan to develop KITs containing several lipases
immobilized in different forms. Development of the so called off-the-shelf
products would change the habitual approach of synthetic chemists since the
use of such a biocatalyst is easier than a synthetic chemical reaction with
the required specifity and selectivity. These
enzymes and enzyme products might be superior in synthetic biotransformations over the commercialized thermophilic fungal lipases with respest
to the degree of enantiomer selectivity or direction/degree of enantiotopic selectivity.
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