Research Team



Useful links



ü Highly stable and active biocatalysts were developed through covalent site-specific immobilization of PcPAL variants (with Cys residues introduced at the enzyme’s surface by site-directed mutagenesis) onto maleimide-functionalized amino-single-walled carbon nanotubes

ü Efficient procedures for the enzymatic synthesis of highly valuable l-phenylalanines in batch and continuous-flow systems were developed by the use of novel site-specifically immobilized biocatalysts

ü Phenylalanine ammonia-lyases from different sources were tailored through rational modification of the hydrophobic binding pocket for the synthesis of a variety of unnatural d- and l-phenylalanines

ü A general rational design strategy for substrate-tailored PALs was proposed


Scientific report Year 1

Scientific report Year 2

Final scientific report


Publications & conferences

1. Krisztina Boros, Mădălina Elena Moisă, Levente Csaba Nagy, Csaba Paizs, Monica Ioana Toşa, László Csaba Bencze, Robust, site-specifically immobilized phenylalanine ammonia-lyases for the enantioselective ammonia addition of cinnamic acids, Catal. Sci. Technol. 2021, 11, 5553-5563, DOI: 10.1039/d1cy00195g.

2. Souad Diana Tork, Mădălina Elena Moisă, Lilla Cserepes, Alina Filip, Levente Csaba Nagy, Florin Dan Irimie, László Csaba Bencze, Towards a general approach for tailoring the hydrophobic binding site of phenylalanine ammonialyases, Sci. Rep. 2022, 12, 10606, DOI:10.1038/s41598-022-14585-0.

3. Mădălina Elena Moisă, Judith-Hajnal Bartha-Vári, László-Csaba Bencze, Florin Dan Irimie, Csaba Paizs, Monica Ioana Toșa, Site-specifically immobilized phenylalanine ammonia lyases for continuous flow processes, The 13the International Symposium of the Romanian Catalysis Society RomCat2022, Băile Govora, 20-24 June 2022 – poster presentation