Web of knowledge for

INOVAMAT

Contract number 241/2014

National Authority: UEFISCDI

UNIVERSITATEA BABES BOLYAI

FACULTATEA DE CHIMIE SI INGINERIE CHIMICA

 

DEVELOPMENT OF INNOVATIVE NANOMATERIALS BASED ON ADVANCED NANOTECHNOLOGY WITH APPLICABILITY IN PROPHYLAXIS OF DENTAL AND PERIODONTAL DISEASES

     

 

 

 

 

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OBJECTIVES

EXPECTED RESULTS

OBTAINED RESULTS

DISSEMINATION

 

 

 

 

 

 

·   Obtained results:

 

Phase 1: Design and realization of innovative nanomaterials for tooth pastes and endodontic cement, experimental models (EM).

 

Summary

A complex documentary study was achieved on the generalities on dental anatomy as well as on the action and the role of nanomaterials with applicability in the prophylaxis of dental and periodontal diseases. A documentary thorough study was realized on cements based on bioceramics, primarily on hydroxyapatite, used in endodontics and on the humans response at the contact with bioceramics and their possibilities of integration.

 

Several preparation methods were designed and realized for INOVAMAT nanomaterials taking into account their synthesis by wet methods and various thermal treatments. Then, their thermal treatment was optimally established and correlated with the wanted crystallinity degree and the size of nano particles, as well as with specific surface area, porosity and biological behaviour in cell cultures. The obtained INOVAMAT nanomaterials were analysed by advanced methods: FTIR, FT-Raman, X-ray diffractions (XRD), and morphological characterization by SEM, TEM and AFM as well as for their thermal stability by TG, DTA and DSC, surface area by BET, composition analysis and mechanical properties.

 

Finally, the most advanced nanomaterials with the proper characteristics are used for tooth pastes, prophylactic pastes and endodontic cement, which will be also characterized from physical, chemical and biological point of view. Our studies indicated that INOVAMAT innovative materials, containing bio-active nano particles with high adsorption capacity on the teeth surface, are strongly integrated in dental enamel and dentine structure.
 

 

Phase 2: Realization and characterization of the obtained innovative nanomaterials for tooth pastes and endodontic cement.
 

Summary

This phase was focused on the realization of innovative nanomaterials for toothpastes and endodontic cements, whose experimental models (EM) have been designed and developed in the previous phase, as well as on the elaboration of prototype models (P). In order to achieve the proposed objectives, in this phase, the experimental study on the action and role of nanomaterials in the prophylaxis of dental diseases was finalized. From this study, it clearly results that using nano hydroxyl phosphate as a re-mineralization and desensitizing agent is promising in the treatment of incipient caries and of dental sensitivity, even though more studies are needed to demonstrate the long-term efficacy. We realized toothpastes and endodontic cement including INOVAMAT nanomaterials.

 

The nanomaterials and their bio-composites with collagen and/or chitosan were characterized by X-ray diffraction (XRD), FTIR (analysis of functional groups), transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy, AFM, and BET surface analysis (specific surface area and porosity). For the toothpastes, the physical, chemical and microbiological characterization was performed, specifying the characteristics and admissibility conditions. The characterization of the endodontic cement was also performed by XRD on powders, AFM, SEM and FTIR. During this stage, investigations were performed in order to establish the relationship between structure, morphology of dental products, INOVAMAT toothpastes and enamel re-mineralization of tooth slices, by AFM and SEM analyses. The biocompatibility of INOVAMAT materials in vitro was evaluated by using various tests of cells viability, such as FDA and Alamar blue assays. The assessment of the mineralization process was performed by alkaline phosphatase activity and immunochemical staining.

 

The results obtained with EM materials have allowed the realization of the preparation methods for INOVAMAT nanomaterials (P). Based on the realized toothpastes (EM), the toothpastes (P) containing INOVAMAT nanomaterials were fabricated; the obtained results give the fabrication standard as well as the qualitative and quantitative composition proposed for the prototypes. Moreover, it was observed that the teeth treatment with the new prepared toothpaste prototypes enhance the surface quality of de-mineralized enamel thus, restoring the mineralized healthy teeth surface.

 

Phase 3: Design and realization of innovative nanomaterials for toothpastes – prototype (P) – first part

 

  Objectives for execution phase:
- INOVAMAT (EM) nanomaterials biocompatibility characterization in the

   cell cultures – second part;
- Elaboration, realization and verification of the INOVAMAT (P) preparing methods,

   second part;
- Elaboration and realization of toothpastes with INOVAMAT (P) nanomaterials,

   second part;
- Physical and chemical characterization of the nanomaterials and prototype and

   tooth paste prototype, first part;
- Results dissemination.
 

  Obtained results

- Report of biocompatibility characterization for P nanomaterials in the cell culture
- Methods of preparation and verification INOVAMAT nanomaterials
- Toothpastes design with INOVAMAT nanomaterials included
- Report of prototype nanomaterials characterization and tooth paste prototype
- Report concerning enamel remineralization
- Dissemination of the obtained results (scientific articles, conference communications).

 

Phase 4: Design and realization of innovative nanomaterials for toothpastes – prototype (P) – second part

 

  Objectives for execution phase:

- Physical and chemical characterization of the nanomaterials and prototype

   and tooth paste prototype, second part;
- INOVAMAT materials (P) – enamel remineralization – second part;
- Biocompatibility characterization of INOVAMAT materials (P) in cell cultures;
- Patent proposal submission. Identification of property rights;
- Results dissemination.

 

  Obtained results

- Report: Prototype materials and toothpaste characterization;
- Report: Enamel remineralization using INOVAMAT materials (P)
- Report: characterization of INOVAMAT materials (P) in cell cultures;
- Patent proposal submission. Identification of property rights;
- Obtained results dissemination: Scientific articles; Communications; Ph. D. Thesis.