|
Web of knowledge for INOVAMAT Contract number 241/2014 National Authority: UEFISCDI |
UNIVERSITATEA BABES BOLYAI FACULTATEA DE CHIMIE SI INGINERIE CHIMICA
DEVELOPMENT OF INNOVATIVE NANOMATERIALS BASED ON ADVANCED NANOTECHNOLOGY WITH APPLICABILITY IN PROPHYLAXIS OF DENTAL AND PERIODONTAL DISEASES
|
|
|
|
· Obtained results:
Phase 1: Design and realization of innovative nanomaterials for tooth pastes and endodontic cement, experimental models (EM).
Summary A complex documentary study was achieved on the generalities on dental anatomy as well as on the action and the role of nanomaterials with applicability in the prophylaxis of dental and periodontal diseases. A documentary thorough study was realized on cements based on bioceramics, primarily on hydroxyapatite, used in endodontics and on the humans response at the contact with bioceramics and their possibilities of integration.
Several preparation methods were designed and realized for INOVAMAT nanomaterials taking into account their synthesis by wet methods and various thermal treatments. Then, their thermal treatment was optimally established and correlated with the wanted crystallinity degree and the size of nano particles, as well as with specific surface area, porosity and biological behaviour in cell cultures. The obtained INOVAMAT nanomaterials were analysed by advanced methods: FTIR, FT-Raman, X-ray diffractions (XRD), and morphological characterization by SEM, TEM and AFM as well as for their thermal stability by TG, DTA and DSC, surface area by BET, composition analysis and mechanical properties.
Finally, the most advanced nanomaterials with the proper
characteristics are used for tooth pastes, prophylactic pastes and
endodontic cement, which will be also characterized from physical, chemical
and biological point of view. Our studies indicated that INOVAMAT innovative
materials, containing bio-active nano particles with high adsorption
capacity on the teeth surface, are strongly integrated in dental enamel and
dentine structure.
Phase 2: Realization
and characterization of the obtained innovative nanomaterials for tooth
pastes and endodontic cement. Summary This phase was focused on the realization of innovative nanomaterials for toothpastes and endodontic cements, whose experimental models (EM) have been designed and developed in the previous phase, as well as on the elaboration of prototype models (P). In order to achieve the proposed objectives, in this phase, the experimental study on the action and role of nanomaterials in the prophylaxis of dental diseases was finalized. From this study, it clearly results that using nano hydroxyl phosphate as a re-mineralization and desensitizing agent is promising in the treatment of incipient caries and of dental sensitivity, even though more studies are needed to demonstrate the long-term efficacy. We realized toothpastes and endodontic cement including INOVAMAT nanomaterials.
The nanomaterials and their bio-composites with collagen and/or chitosan were characterized by X-ray diffraction (XRD), FTIR (analysis of functional groups), transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy, AFM, and BET surface analysis (specific surface area and porosity). For the toothpastes, the physical, chemical and microbiological characterization was performed, specifying the characteristics and admissibility conditions. The characterization of the endodontic cement was also performed by XRD on powders, AFM, SEM and FTIR. During this stage, investigations were performed in order to establish the relationship between structure, morphology of dental products, INOVAMAT toothpastes and enamel re-mineralization of tooth slices, by AFM and SEM analyses. The biocompatibility of INOVAMAT materials in vitro was evaluated by using various tests of cells viability, such as FDA and Alamar blue assays. The assessment of the mineralization process was performed by alkaline phosphatase activity and immunochemical staining.
The results obtained with EM materials have allowed the realization of the preparation methods for INOVAMAT nanomaterials (P). Based on the realized toothpastes (EM), the toothpastes (P) containing INOVAMAT nanomaterials were fabricated; the obtained results give the fabrication standard as well as the qualitative and quantitative composition proposed for the prototypes. Moreover, it was observed that the teeth treatment with the new prepared toothpaste prototypes enhance the surface quality of de-mineralized enamel thus, restoring the mineralized healthy teeth surface.
Phase 3: Design and realization of innovative nanomaterials for toothpastes – prototype (P) – first part
Objectives for execution phase:
cell cultures – second
part;
second part;
second part;
tooth paste prototype, first
part; Obtained results
- Report of biocompatibility characterization for P
nanomaterials in the cell culture
Phase 4: Design and realization of innovative nanomaterials for toothpastes – prototype (P) – second part
Objectives for execution phase: - Physical and chemical characterization of the nanomaterials and prototype
and tooth paste prototype, second part;
Obtained results
- Report: Prototype materials and toothpaste
characterization; |