Csaba started the project at the application level in 2015 and hopes he survives successfully all the five years of the project. Fortunately the highly motivated and competent members of his earlier RU-TE project continued with him on the PROMYS, providing a proper start of the project. He believes in his team and in the project and hopefully the result will confirm him.

Author identifiers:

ResearchGate

Kati helps us with theoretical investigation of the chemical and biological processes. She has experience with computational quantum chemistry and bioinformatics.

Raluca joined us during her diplomawork, and now is her first year of Msc. studies. As biochemical engineer she is interested in protein engineering using semi-rational tehcniques, such as ISM or CASTing. Currently she works on improving the thermostability of MIO-enzymes.

Krisztina as biochemical engineer student in her 4th year of studies, joined us in 2017 and works intensively on the challenging site-specific immobilization of MIO-enzymes. Hopefully she remains with us also for Msc. studies!

Horia as biochemical engineer student in his last, 4th year of studies, joined the Biocatalysis Research Center several years ago,from his first year of studies and by working on various PAL-related topics he accumulated diverse skills in enzyme-related topics. Recently he joined the sub-project related to the substrate scope extension of FDC1.

Ilka as 2nd year Bsc. student joined our group for her diplomawork in 2016 and works intensively on developing novel protease and heat-stable PcPAL mutants. She is interested in molecular cloning, enzyme assays, enzyme rational design, representing our future generation.

Mada joined the Biocatalysis and Biotransformation Research Group in 2011 as an undergraduate student in Biochemical Engineering, while working for her bachelor thesis. Since then, she is with the group, currently as PhD student in her last year under the supervision of Prof. Dr. Monica Ioana Toşa. Meanwhile she accumulated large experience in chemoenzymatic procedures, enzyme immobilization, organic synthesis, isolation, purification and spectral analysis of organic compounds (NMR, MS), chiral high performance liquid and gas chromatography, design and control of biocatalytic processes. In this year she joined the project with the aim to continue the work with HTS-assays and to develop the site-specific immobilization. We are very happy to have her in our group.

Ph.D student Filip Alina as biologist joined the Biocatalysis and Biotransformation Research Center at 2013, when she started her Ph.D work under the supervision of Prof. Dr. Florin Dan Irimie. In 2014 she joined the RU-TE project, working on MIO-enzymes and subsequently the PROMYS project. Her skills and competences are: molecular cloning, expression, isolation and purification of proteins, site-directed mutagenesis, electrophoresis techniques, enzyme kinetics, enzyme assays. In 2017 Alina was granted with an assistant professorship, being responsible for laboratory courses of Basic and Advanced Biochemistry.
She likes poetry, painting and her work. "If you do tomorrow what you did today, you will get tomorrow what you got today".

Souad-Diana Tork joined our research team in 2013, in her second year of study of the B. Sc. During these years she managed to obtain several scholarships, including World Federation of Scientist (WFS) Scholarships and Scientific Performance Scholarship working on the research which she continues also on her Ph.D study: Novel mutant MIO-enzymes for the synthesis of unnatural amino acids.
Diana has made conspicuous progress by assimilating basic techniques used in our laboratories such as: overexpression of recombinant proteins in Escherichia coli, purification and analysis of such proteins (Ni-NTA affinity chromatography, SDS-PAGE electrophoresis, Western Blot), UV-vis spectroscopy, HPLC spectroscopy, TECAN Spark® multimode reader, primer design (site-directed mutagenesis), PCR (polymerase chain reaction ), DNA isolation.
Diana is now a Ph.D student under the supervision of Prof. Eng. Florin-Dan Irimie and is committed to permanently improve her practical capabilities and theoretical knowledge.

Andi currently joined the PROMYS project, but has several year experience on MIO-enzymes, also her Ph.D work performed within the Biocatalysis and Biotransformation Research Center was based on PAL and PAM mediated biotransformation. Despite she helps us out on short-term on our vacant postdoc position, we can fully exploit her knowledge and experience in enzyme kinetics, molecular cloning, enzyme isolation and purification techniques.

Nora joined our group in 2016, as microbiologist postdoctoral fellow and worked on HTS-assay development for PAL-activity screens and the generation of mutant PAL libraries. Her experience accumulated during her Ph.D work in Hungary helped us a lot in solving microbiological issues.

Boti as Ph.D student in the Biocatalysis and Biotransformation Research Center, joined the project in his last year of Ph.D studies and helped us with substrate synthesis and NMR measurements.

Zsofi joined our group in 2014 as undergraduate student, and since 2015 she is working on the MIO-enzyme projects, first in the RU-TE project, where he contributed significantly to the substrate scope extension of PcPAL and since October 2017 she started her Ph.d on the PROMYS project where she works on HTS-assay development and FDC1 substrate scope characterization. She likes to work, to travel home and to share words with others and all of us are very glad to have Zsofi in our team.

Diana joined us during her diplomawork, and now is her first year of Msc. studies. As biochemical engineer she is interested in genetic engineering, molecular cloning of enzymes, enzyme isolation and purification and kinetic studies. Currently she works on generation novel mutant MIO-enzymes with enhanced catalytic properties. Besides her research work, Diana is very involved in student organisations, and as student representative at different councils.

Diana Amariei currently is in her 4th year of bachelor studies at Biochemical Engineering specialization. She is working on the development of high-throughput activity assay for PALs, with the aim to learn all there is to learn, being interested in genetic engineering, proteomics and microbiology. Her work includes enzyme kinetics (through spectrophotometric and fluorometric methods), primer design, protein purification and mutant cells transformation and screening. Her not-so-academic passions include astronomy, visual arts, travelling and rock music.