Infrastructure

The infrastructure of the research center has been intensively developed in the last years, through the financial support of several national and international research projects of the research group members. Furthermore several instruments were obtained by donations from our long-time collaborators, Terapia Ranbaxy, Farmec, Kynetix.

The infrastructure is organized over several laboratories:

  1. Synthesis labs (L.11 and L.16)
  2. Fermentation/protein purification labs (L.6 and L.36)
  3. Protein engineering and HTS-screening lab (L.32 and L.33)
  4. Bioanalytic lab with LC-MS analysis platform (L.34)
  5. Immobilization and continuous-flow reactor lab (L.53)
  6. Protein crystallization lab (L.9)
  7. Computational facilities

The research center also has access to the strategic research facilities of the Faculty of Chemistry and Chemical Engineering of Babes-Bolyai University (NMR spectrometers. Atomic absorption-emission spectrometer, etc.).

Regulation for the use of biotransformation and biocatalysis research center equipment.

Equipment list

CFX96 Touch™ Real-Time PCR Detection System

Manufacturer: Bio-Rad
The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

Technical description

96-well PCR system, Mastercycler ProS

Manufacturer: Eppendorf
The Mastercycler ProS allows us to perform gradient PCRs in 96-well plate format.

Technical description

Prometheus NT.48

Manufacturer: NanoTemper Technologies GmbH 2016
Differential Scanning Fluorimeter which allows the high-throughput determination of protein stabilities, based on the detection of the intrinsec fluorescence of Trp and Tyr residues of proteins (sample concentration range: 5 µg/ml - 250 mg/ml).

Technical description

Transmission electron microscope TEM Hitachi H-7650

Manufacturer: Hitachi Group 2010
Registration of high resolution and contrast images, necessary for analytical, biomedical, materials sciences.

Technical description

Protein purification system FPLC-Äkta

Manufacturer: GE Health Care 2007
Pump with two channels, detector UV 900, conductometer, fraction collector Frac-950, Unicorn software.

Technical description

Microplate reader - TECAN 10M Spark

Manufacturer: Tecan Group Ltd.
Versatile microplate reader, capable of measuring fluorescence, absorbance, and luminescence, as well as cell confluency and cell number.

Technical description

Lyophilizer - ALPHA 1-2 LDPlus

Manufacturer: Martin Christ 2007
Equipped with high-vacuum (< 0.040 atm) pump

Technical description

Electrospinning system FLUIDNATEK

Manufacturer: Bioinicia
A precise electrospinning and electrospraying system to produce nanofibers and particles with tightly controlled diameters and morphologies. It offers fully adjustable parameters including emitter and collector voltages, flow rate, automated needle translation, spinning distance, and more for optimized nanomaterial production.

Technical description

Agilent LC-MS system

Manufacturer: Agilent Technologies Inc. 2006
Analysis and characterization of compounds, inclusive from a complex mixture (biological samples). Equipped with specific software and databases as well as the necessary auxiliar instrumentations.

Technical description

Chemidoc imaging system

Manufacturer: BioRad

Technical description

Flow chemistry system - SpinSplit

Manufacturer: SpinSplit Technical Research and Development LLC

Technical description

Ultrasonic Microwave Chemical Reactor UMCR-SM50

Manufacturer: Col-Int Tech (USA), LLC

Technical description

Chiral chromatographic analysis unit

HPLC Agilent 1150 (2002)
  • Quaternary pump, degasor
  • DAAD detector
  • Chemstation software
  • autosampler
HPLC Agilent 1200 (2006)
  • Quaternary pump, degasor
  • DAAD detector
  • Chemstation software
HPLC Agilent 1260 (2012)
  • Quaternary pump, degasor,
  • DAAD detector
  • ELSD detector (with N2 generator)
  • Chemstation software

A collection of standard and chiral columns (> 30).

Technical description: 1150, 1200 and 1260 model.

Gas chromatograph GC Agilent MF 235330

Manufacturer: Agilent Technologies Inc. 2009
FID detector, autosampler, Chemstation software –equipped with diverse standard and chiral columns.

UV-VIS Agilent 8453 spectrophotometer

Manufacturer: Agilent Technologies Inc. 2010
Analysis in UV-VIS domain, equipped with thermostat, multi-channel sample system with 8 positions and specific software including enzyme kinetics and other applications.

UV-VIS spectrophotometer Selecta 3100

Manufacturer: Selecta 2016
Analysis in UV-VIS domain, specific software including enzyme kinetics and other applications.

Bioreactor MS-F1

Manufacturer: Bionet Engineering Co 2010
Autoclavable 4L bioreactor, equipped with pums, filters, specific sensors / electrodes and software for control.

Termocycler Eppendorf Mastercycler Pro

Manufacturer: Eppendorf Co. 2007
Thermocycler with 24 positions, useful for PCR amplification procedures of DNA samples.

Horizontal and vertical electrophoresis systems with gel-casting

Manufacturer: Biorad 2015, 2016
Preparation and migration of 1-4 gels used for the analysis of proteins and nucleic acids.

Microstar 17R microcentrifuge

Manufacturer: VWR Co. 2015
Max. 13000 rpm, 24 position, with cooling system.

Vertical shaker Biosan Multi Bio RS-24

Manufacturer: Biosan 2015
5-30 rpm, vertical rotations of 360°.

Transiluminator- UVP Bioimaging

Manufacturer: UVP Analytic Jena 2015
Filters for λ = 300, 302, 312 nm useful for detection of DNA in gels with dimensions of 15x15 to 25x26 cm.

Titramax 1000 shaker

Manufacturer: Heidolph 2014, 2016
32-48 sample positions, 150-1350 rpm, adjustable time range: 1-120 min, temperature range 20 - 65°.

Vibramax 100 shaker

Manufacturer: Heidolph 2009, 2015
150-1350 rpm, adjustable time range: 1-120 min.

Incubator Multi Stack Shaking Labtech

Manufacturer: Daihan Labtech India Pvt. Ltd. 2007
100-300 rpm, 25-70°C, 10-2000 mL glassware.

Incubator Innova 40 R

Manufacturer: New Brunswick 2016
25-500 rpm, temperature range 25-70 °C, 10-2000 mL

Raypa AES-40 autoclave

Manufacturer: Raypa Lab. Equipment 2007
40 L, with various predefined sterilisation programs.

Presoclav 80 autoclave

Manufacturer: Selecta 2016
40 L, with various predefined sterilisation programs.

Titrator 902 Titrando – pH STAT

Manufacturer: Methrom 2012
pH measurements, automated titration, pH monitoring and automated adjustment, 3 point calibration, etc.

ADP220 Digital polarimeter

Manufacturer: Bellingham Stanley 2005
with cuvettes of 1,2 and 10 ml.

GFL Water Still

Manufacturer: Jena 2015
System for double-distilled water with a production capacity of 2 l/h.

Deep-freezer Premium U410 Ultra-Low Temperature

Manufacturer: New Brunswick 2014
-81 °C, volume of 80 L.