WP1. Recombinant production of various PET hydrolyzing enzymes (M1-M6)

T1.1. Molecular cloning and site-directed mutagenesis (M1-M3)

T1.2. Expression, isolation and purification of PET-hydrolases (M4-M6):

T1.3. Functionality tests of the purified PET-degrading enzymes (M4-M6)

WP2. PET-degrading activity of multienzymatic systems including PETases, lipases, and esterases (M3-M12)

T2.1. PET-degrading activity screens for selection of best performing enzyme mixtures

T2.2. Process optimization for the best performing enzyme combinations

WP3. Development of optimized co-immobilization strategies of PET-degrading enzymes (M9-M21)

T3.1. Preparation of functionalized supports (M9-M14):

T3.2 Immobilization of PET-degrading enzyme combination with covalent binding and adsorption (M14-M16)

T3.3 Sequential co-immobilization (M17-M18)

T3.4. Co-expression and affinity ‘fishing’ based co-immobilization (M17-M18)

T3.5. Site-specific immobilization version of the best-perfoming covalent immobilization system

WP4. Development of immobilized PET-degrading enzymes for micro(nano)-PET removal (M21-M34)

T4.1. Testing the microPET-degradation efficiency selected immobilized multienzymatic systems in batch conditions (M21-M24)

T4.2. Testing the immobilized multienzymatic systems in continous flow procedures (M25-M30)

T4.3. Testing the operational stability, recyclability of the immobilized multienzymatic systems on different environmental water samples with microplastic content (M31-M34)

WP5. Development of immobilized PET-degrading enzymes for micro(nano)-PET detection (M21-M36)

T5.1. Fine-tunning the immobilization on nanosupports (M21-M25):

T5.2. Assembly of multienzymatic microfluidic systems (M26-M32):

T5.3. Assembly and validation of nanofiber immobilized multienzymatic systems (M33-M36)

WP6. Project management including dissemination, communication, exploatation activities (M1-M36)